Label-free Quantification of Phosphorylation Dynamics in PDGF-stimulated NIH 3T3 Wild Type and MEK1-depleted Cell Lines

摘要

By utilizing a robust IMAC-LC/MS/MS method, over 2,000 phosphopeptides were quantified. Differences in the phosphorylation dynamics between the two cell lines indicate that MEK1 is an essential protein involved in multiple pathways. By spiking in AQUA peptides, increased levels of ERK2 phosphorylation in the shMEK cell line were quantified, suggesting that MEK2 could be compensating for MEK1.