QUANTIFICATION OF QUERCETIN AND STIGMASTEROL OF COUROUPITA GUIANENSIS AUBL BY HPTLC METHOD AND IN-VITRO CYTOTOTOXIC ACTIVITY BY MTT ASSAY OF THE METHANOL EXTRACT AGAINST HeLa, NIH 3T3 AND HepG2 CANCER CELL LINES

摘要

The methanol extract of the dried flower of Couroupita guianensis was subjected to the HPTLC analysis. Further the extract was screened for cytotoxicity against the HeLa, NIH 3T3 and HepG2 cancer cell lines. It showed significant cytotoxicity with IC5 0values of 202.6, 280 and 470.8 μg/ml towards HeLa, NIH 3T3 and HepG2 cancer cell lines respectively. Two compounds stigmasterol (I) and quercetin (II) were isolated from the methanol extract and identified by their IR, NMR and Mass spectral data. Further I and II were quantified by HPTLC methods (242.14 μg/ml and 188.97 μg/ml respectively). II was identified as the biomarker compound