Proteomic Analysis of Mouse Macropha ge (RAW264.7) Cells Infected With Burkholderia pseudomallei K96243

摘要

An MS-based proteomics approach was used to characterize macrophage host cell response to B. pseudomallei infection. In response to the bacterial infection, ～80 and ～120 host cell proteins were found up-regulated and down-regulated, respectively. Bioinformatics analysis of host cell response indicates that the suppressed proteins mainly involves in bio functions including nucleic acid binding and mRNA processing, which suggests an impact on protein translation. Up-regulated host cell proteins mainly involves in bio functions including protein folding and cell signaling. Figure 1 shows the top overlapping canonical pathways the significantly impacted host-ce ll proteins are involved in. To understand the underlying molecular mechanism of the B. pseudomallei interaction with RAW264.7 cells, upstream regulator analysis using Ingenuity pathway determines ABP1 and E2F4, both transcription fa ctors, as the most significant upstream regulators of up-regulated and down-regulated host proteins upon infection, respectively. ABP1 recently has been reported to pl ay a role in autophagy signaling (2) and E2F4 has been known to play a role in gene expression repression. Besides investigation of the host-cell response, 160 bacteria proteins were also identified in the infected RAW 264.7 cells in this study. The bacteria proteins identified include proteins Hcp, IcmF, ClpV (all belong to type VI secretion system) as well as signal response particle receptor FtsY from K96243 KEGG bacteria secretion pathway. Further functional annotation clustering analysis of the identified bacteria proteins indicates high enrichment for proteins involving in biological processes including translat ion and TCA cycle. The data analysis is still on-going and should provide us insight into the bacteri a virulence as well as its pathogenicity.