Mass Spectrometry Analysis and Imaging of Lipids and Proteins in Human Liver via MALDI and Liquid Extraction Surface Analysis

摘要

Liver disease is one of the five biggest killers in the UK [1]. Liquid Extraction Surface Analysis (LESA) has previously been used in analysis of TLC plates, dried blood spots, whole animal sections and fresh tissue [2-4]. In this work we explore the use of LESA coupled to high resolution nano ESI-MS and MS/MS for analysis of lipids and proteins in human liver tissue.