Fast and Clean Sample Preparation With No Dry Down Steps for Peptide Mixture Extraction From Plasma Prior to LC-MS/MS Analysis.

摘要

Proteomics has become one of the foremost bioanalytical fields directed towards the discovery, detection and characterization of proteins. An integral part of this analytical process typically involves some level of sample pretreatment due to the size and complexity of proteins and the matrix components which can interfere with detection at low levels. A common pretreatment approach is digestion of a protein to characteristic peptide fragments prior to mass spectral analysis. Methodologies for subsequent extraction of these peptides from biological matrices can be time consuming and ineffective. This poster outlines a new method for the extraction of a diverse mixture of peptides from plasma using a solid phase extraction polymer resin prior to LC-MS-MS.