Proteomic Biomarker Analysis of Acetaminophen-induced Hepatotoxicity in Rats

摘要

Drug-induced liver injury (DILI) is an adverse event that frequently leads to cessation of drug testing in clinical trials, restrictions on drug use, and the withdrawal of approved drugs. Existing biomarkers of liver injury (e.g., serum alanine and aspartate aminotransferases [ALT and AST]) lack needed specificity. Bilirubin has the required specificity but only is altered after functional change when it may be too late. New biomarkers of high specificity and sensitivity for hepatotoxicity detection are needed. Acetaminophen (APAP) overdose is a major cause of DILI. To understand the molecular mechanisms underlying APAP-induced hepatotoxicity and identify associated biomarkers, a quantitative proteomic analysis was conducted on livers from APAP-treated rats.