Detection of Detection of Angiotensin II and Insulin by Using Graphene Matrixes in Surface-Assisted Laser Desorption/Ionization Mass Spectrometry

摘要

In this study, we demonstrated that use of graphene as a high efficiencypreconcentrationand detection platform forbiomoleculesfrom complex biological matrices. Biomolecules-adsorbed graphene can be used directly for Surface-assisted Laser Desorption Ionization-Time of Flight-Mass Spectrometry (SALDI-TOF-MS). First, we conductedfourier transform infrared spectroscopy(FT-IR), Raman measurements, and thermal gravimetric analysis to confirm the composition of the as-prepared graphene. This approach provided linearity for angiotensinII, insulin and CytochromeC over the concentration range of 0.5-500nM(R2 = 0.9947), 0.5-500nM(R2 = 0.9911) and 20-500μM(R2 = 0.9997), with a limit of detection (signal-to-noise ratio = 3) of 0.15, 0.11 and 7.5nM, respectively. We have validated the practicality of thegraphenefor Surface-assisted laser desorption/ionization mass spectrometry of biomolecules.