Dynamics of Transferrin Receptor and Interactions Critical for Binding Transferrin Studied by Hydrogen Deuterium Exchange Mass Spectrometry

摘要

1. HDX-MS can be used to identify changes in conformational dynamics upon TfR binding to Tf. 2. Confirmed regions located at the crystallographically identified Tf/TfR interface show more protection upon Tf binding. 3. Identified new regions located within the protease-like domain of TfR, close to the predicted Tf binding region, that become more protected upon Tf binding. 4. Several peptides close to the protein surface, based on the recently solved crystal structure, demonstrate high level of protection, even in TfR alone.