DEVELOPMENT OF A SENSITIVE METHOD FOR QUANTITATION OF 6-HYDROXIMINO-4-AZA-A-HOMO-CHOLEST-3-ONE IN MOUSE PLASMA USING LC-MS/MS

摘要

The method was calibrated for a concentration range from 0.500 to 200 ng of Hym3 per mL of plasma. By measurement of quality control samples prepared by spiking HyM3 and the internal standard into plasma at three concentrations (low, medium, and high), we found that this method was precise (CV < 10%) and accurate (the error < 15%). The low limit of quantitation (LLOQ) was 0.500 ng/ml. Some plasma samples from HyM3-administrated mice were analyzed, and the results have showed that the method is applicable for such measurement.