Detection of modified ribonucleotides from native RNA using negative ion mode ESI MS coupled to nanobore ion pair reagent LC

摘要

Here we set up a general approach to the straight-forward analysis of the (i) Nucleotide content of RNA and DNA preparations, (ii) quality control of such samples, (iii) detection of endogenous (e.g. 5m-CMP) and sample preparation induced (e.g. ox-AMP) modifications and the localization of this modification either to the nucleobase or the backbone (e.g. the ribose). The ion pair reagent based separation on micro-bore columns enables the sensitive detection and quantification of different mononucleotide species and their quantification by negative ion pair reagent LCMS.