Observation of Anti-Cancer Drug Influence to Ganglioside Profiles Using 15 T FT-ICR MS

摘要

We found that great changes in the lipid composition of gangliosides were induced by application of anticancer drugs (4-HPR, camptothecin, and tamoxifen) by analysis of nano-LC/FT-ICR MS. Typically, GM3 and their oxide forms were mainly observed and greatly increased by anti-cancer drugs, indicating that oxidative stress and resultant reactive oxygen species (ROS) may be involved to trigger membrane damage and apoptosis of U87MG cells by drugs treatment. Especially, GM3 was profoundly increased compare to other gangliosides like GM1, GD1 and GD3. These increases of membrane gangliosides suggested that GM3 could have a strong therapeutic potential against tumor cell growth and angiogenesis with an agonistic or antagonistic combination of other gangliosides. In spite of the complexity of biological mixture of lipids, relative abundance profiling and their formula identifications of the polar lipids can be possible without any difficulties by the ultra-high resolution 15 TFT-ICR MS.