Study of Prostate-Specific G Protein-Coupled Receptor Signalling in human LNCaP cells

摘要

a We successfully demonstrated that our phosphoproteomics strategy is well suited to identify phosphopeptides from orthovanadate-treated LNCaP cells. We were able to identify 2569 phosphorylation sites in 2095 phosphopeptides using as little as 200 μg of peptides in each of the two experiments. a Two different analysis platforms have been tested. Both platforms gave complementary results, while the Orbitrap/MaxQuant workflow performed best in respect to the amount of peptides and to the localisation site assignment. a A quantitative time course study of beta-ionone treated LNCaP cells resulted in the detection of 52 differentially regulated proteins. Amongst these proteins were components of the calcium homeostasis machinery as well as proteins associated with migration and cellular movement.