SILAC and label-free approaches for quantitatively identifying caveolae proteome

摘要

Both SILAC and label-free quantitation are useful in subcellular proteomics. For quantitative organellar proteomics the choice of procedure can lead to unintended bias in the data if the proteomes being compared are normalized too late in the procedure. Therefore, potential differences in the relative abundance of the organelle/fraction between samples need to be considered during experimental design and data interpretation.