Increased Ion Sampling Efficiency Allows Streamlining Sample Preparation for the Determination of Fluticasone Propionate in Human Plasma

摘要

Taking full advantage of 6490 Triple quadrupole increased sensitivity, it was possible to streamline the workflow by relaxing the requirements on sample preparation. Sensitivity of the instrument was demonstrated with LLOQ at 5 pg/mL, corresponding to 2.5 fg abs. injected on column. The new enhanced resolution capability of the instrument may be beneficial in eliminating interference noise and therefore increasing specificity. Assay performance (correlation coefficient for calibration curves, precision and accuracy) under unit/unit and enhanced/unit resolution were similar and both within current pharmaceutical and regulatory guidelines.