The Clp protease complex subunit stoichiometry in Arabidopsis thaliana chloroplasts determined by quantitative mass spectrometry using QconCAT technology

摘要

A Clp-QconCAT protein consisting of two proteotypic peptides representative of each protein in the plastid- localized Clp protease family was purified and can obtained in labeled or unlabeled form. H/L ratios within two orders of magnitude can be obtained for several of the peptides at femtomolar levels. MS analysis needs to be further optimized.