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Development of a Novel Mass Spectrometry-Based Method for Simultaneous Qualitative and Quantitative Protein Citrullination Analysis of Complex Biological Samples
Development of a Novel Mass Spectrometry-Based Method for Simultaneous Qualitative and Quantitative Protein Citrullination Analysis of Complex Biological Samples
The present invention discloses mass spectrometry (MS)-based methods and tagging reagents for qualitative and quantitative analysis of post-translational modified (PTM) proteins in biological and clinical samples. The present invention utilizes thiol-containing tagging reagents which are able to bind to or derivatize biomolecules, preferably post-translational modified (PTM) polypeptides such as citrullinated and homocitrullinated polypeptides. The tagging reagents are preferably biotin-derived tags able to react with a side chain of the modified polypeptides.
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