Anti-inflammatory effect of an Escherichia coli extract in a mouse model of lipopolysaccharide-induced

摘要

To evaluate the anti-inflammatory effect of E. coli extract, we measured the cytokine levels of bladder tissue after oral administration and analyzed bladder inflammation by histopathologic examination in a model of lipopolysaccharide-nduced cystitis in mice. After oral administering the E. coli extract for 10 days, the cytokine levels present in the bladder of female Balb/C mice were determined using a cytometric bead array. The bladder macrophage inflammatory protein-2 level was also measured using a sandwich enzyme immunoassay. After immunization with the E. coli extract, E. coli LPS was instilled into the bladders intravesically. Twenty-four hours later, the mice were sacrificed and the level of bladder inflammation was quantified using the bladder inflammatory index (BII). Significant changes in the bladder IL-6 and IFN-c levels were observed after the E. coli extract treatment. E. coli extract treated mice exhibited mild inflammation of their bladders with a significant reduction in the BII scores compared with the controls.