Exenatide Rescues Sirtl Expression in Aβoptotic RINm-5F Cells

摘要

Exenatide is a 39 amino acid peptide which has been used to treat type 2 diabetes. Aside from its clinical application, detailed molecular mechanisms are not fully understood yet. Sirtl is a class III histone protein deacetylase, which is essential in the aging process. It has been reported that Sirtl is involved in glucose metabolism and can improve insulin sensitivity by repressing PTP1B [1]. Furthermore, it is suggested that Sirtl can regulate insulin secretion by repressing UCP2 in pancreatic (3 cells [1]. We hypothesize that exenatide may regulate glucose metabolism through Sirtl. Here, we chose to use RINm-5F cells (an insulinoma cell line) as cell model, induced the cells into apoptosis state and treated the cells with exenatide. We found that sirtl gene expression is decreased under the induction of apoptosis and exenatide is able to rescue the sirtl expression in both gene and protein level.