Micronization of Ganoderma lucidum extract obtained by pressurized hot-water extraction

摘要

Ganoderma lucidum is a mushroom-forming white rot fungus. Though it isn't edible in the traditional sense (for food) it is grown commercially for use as a medicinal supplement. This species has been a part of traditional Eastern medicine for thousands of years. G. lucidum contains a wide variety of bioactive components. The major bioactive polysaccharides isolated from Ganoderma species are glucans, β-1-3 and β-1-6 D-glucans. β-gfucan is able to isolated by extraction. The G. lucidum extract is usually obtained by liquid-solid extraction and sold in powder form. Recently, the extraction and powder formation process has been carried out separately, for example by hot water extraction and spray dryer. This process may cause a problem due to the water content in the extract. The extract may become corrupt. Moreover, it needs more heat to form the extract powder. The energy needed for the process can be reduced by connecting both the hydrothermal and spray dryer process. In this work, connection of G. lucidum pressurized hot-water extraction and micronization of the extract using spray dryer method was studied. Effect of extraction temperature on the formation of particle was examined. Pressurrized hot-water extraction was carried out in a semi-batch at pressures of 1.5 to 4 MPa and temperatures of 160 to 190°C with flow rate of 0.2 mL/min. Spray dryer method was carried out using air at temperature of 170°C. Generated particles were observed by scanning electron microscope (SEM) and molecular weight distribution was analyzed by MALDI-TOF/MS. Morphology of micronized particles was shriveled-sphere-like particle. Particles size and molecular weight distribution were influenced by extraction temperature. Higher molecular weight was obtained at high temperature.