Enzyme Immobilized Biosensor for L-Lactate

摘要

L-lactate has an important role on biochemical process such as producing ATP, causing cramps in muscle. The lactate consumption in various proposes is increasing in human life. It is produced by fermentation process with microorganism. Measuring L-lactate concentration by a rapid and simple method is a great need in clinical and food industries. Analysis of enzyme reaction on L-lactate was performed with L-LDH (L-lactate dehydrogenase), and NAD* (nicotinamide adenine dinucleotide). To avoid enzyme reverse reaction of L-LDH, GPT (glutamic-pyruvic transaminase) and L-glutamate were supplied. Substances were immobilized with polyacrylamide gel on tip of glass electrode. Calibration with L-lactate and output signal was performed. The relation between the L-lactate (%) and the signal from the electrode (mV) was y=0.0719x-16.576. The calibration had a correlation coefficient of R~2=0.9959 and R~2=0.9919 when it was validated.