DEVELOPMENT OF ISOLATION TECHNIQUES AND CAPILLARY ELECTROPHORESIS MASS SPECTROMETRY (CESI-MS) METHODS FOR THE CHARACTERISATION OF NANOMATERIAL PROTEIN CORONAS

摘要

Since the nanoparticle corona rose to eminence a decade ago, it has been investigated by a wide range of researchers trying to characterise and derive its significance [1]. To date, LC-MS is the instrument of choice for the analysis of the protein corona [1,2]. However, CESI-MS offers an exciting prospect to increase sample throughput and separate and detect highly polar and very large peptides which may be missed by conventional nLC-MS platforms [3].