Reproducible quantification of the yeast proteome using data independent acquisition (DIA) mass spectrometry

摘要

Overlapping wide-window DIA method allows for experimental scale equal to discovery DDA experiments, with validated quantitation of thousands of peptides. A label-free response curve enables assessment of 1,000's to 10,000's of target analytes, including precursor measurement reproducibility and stability at 4°C, lower limit of quantitation, and linearity. Median reproducibility of quantitative precursors, as measured by %CV over 72 hours, was 9.51%, with a majority of peptide %CV falling below recommended 20% CV standards.