METHOD FOR MASS SPECTROMETRIC DETECTION OF PROTEOME USING PULSED DATA INDEPENDENT ACQUISITION

摘要

A method for mass spectrometric detection of a proteome using pulsed data independent acquisition, comprising the following steps: (1) configuring M large windows, the interval width of the mass-to-charge ratio of the large windows being 2-150 m/z; (2) evenly dividing each large window into N small windows, the interval width of the mass-to-charge ratio of the small windows being 2-75 m/z; (3) evenly distributing the M*N small windows to N mass spectrometry acquisition methods for scanning, and for each acquisition method, selecting and fragmenting all ions in the M small windows, detecting all fragments generated by the irons in the windows, and obtaining acquisition data; and (4) merging the M*N pieces of acquisition data in the N mass spectrometry acquisition methods in step (3), and analyzing the obtained acquisition data. The method reduces mutual effects of precursor ions formed by polypeptides, so that the resolution of acquired data is improved, and the quantity of protein identifications is increased.