定量蛋白质组学质谱采集技术进展

摘要

Mass spectrometry is an important and powerful tool for protein quantification. With the in-depth development of quantitative proteomics, limitations of classic MS based quantification methods, such as complicated matrix interference and throughput/capacity limitation, start to appear. Recent progress of series novel MS based techniques provide effective solutions for the limitations of relative and absolute proteomic quantification, including synchronous precursor selection ( SPS ) , mass defect isobaric labeling, parallel reaction monitoring ( PRM) , multiplexing acquisition ( MSX) , and various novel data independent acquisition ( DIA) modes. Here we summarized the current limitations of quantitative proteomics, reviewed the latest MS based quantification approaches, and discussed the features and advantages of these novel techniques for quantitative proteomic application.%质谱是定量蛋白组学的主要工具。近年来随着定量蛋白质组学研究的深入，传统质谱定量技术面临着复杂基质干扰、分析通量限制等诸多问题。而最近一系列质谱新技术的发展，包括同步母离子选择( SPS)、质量亏损标记、平行反应监测(PRM)、多重累积(MSX)和多种全新数据非依赖性采集(DIA)等，为解决目前蛋白质组学在相对定量和绝对定量分析方面的局限提供了有效途径。本文对定量蛋白质组学目前遇到的瓶颈问题进行了分析，总结了质谱定量采集技术的最新进展，并评述了这些新技术的特点以及在定量蛋白质组学应用中的优势。