The epitranscriptomics of long noncoding RNAs in S. cerevisiae.

摘要

Cell exposure to environmental stress results in immediate adaptation based on regulatory feedback and cellular memory to maximize survival. The latter involves epigenetic events that can be substantiated by specific DNA methylation, histone modification and, as recently discovered, expression of long noncoding ribonucleic acids (lncRNAs). Natural RNA display >100 variants of the canonical bases, the significance of which is still not understood. Owing to their enzymatic biogenesis, we hypothesize that these post-transcriptional modifications (PTMs) are essential communication nodes between signaling and expression processes, which participate in modulating RNA function. To test this hypothesis, we are employing MS analysis to profile RNA modifications in S. cerevisiae and investigate their role in stress response mediated by lncRNAs.