Urinary metabolomics analysis reveals the impact of surgical menopause in rats on metabolism of endogenous metabolites, and of a dietary supplement, grapeseed extract

摘要

Metabolomics analysis using XCMS and METLIN software enabled identification of ions that were different in rat urines due to OVX, both endogenous steroids and prostaglandins and a bacterial metabolite of a lignan. However, many of the other metabolites that were significantly changed could not be identifed in the METLIN database. Targeted analysis by LC-MRM MS of the same urines indicated that GSE polyphenols are glucuronidated to a greater extent in OVX vs sham-OVX. Since glucuronidation is IN GENERAL a mechanism by which cells excrete xenobiotics, these results imply that the menopausal condition may involve a requirement for higher doses of the same dietary supplements such as GSE for the same bioactivity.