ANALYSIS OF DRUG METABOLITES IN BIOLOGICAL FLUIDS USING MIXED-MODE SOLID PHASE EXTRACTION AND ULTRAPERFORMANCE LIQUID CHROMATOGRAPHY-TANDEM MASS SPECTROMETRY

摘要

A method for extraction and analysis of opiates and their glucuronide metabolites was developed using Oasis~R MCX (mu)Elution SPE and UPLC~(~R)-MS/MS. All analytes were stable throughout the extraction and analysis procedure. Average recovery for all compounds was greater than 93percent over several days, and varied less than 6percent. The 2 minute UPLC~(~R)-MS/MS analysis is suitable for high throughput bioanalysis.