Mercapturic Acid Conjugates of 4-Hydroxy-2-nonenal and 4-Oxo-2-nonenal Metabolites in a Rat Model of Oxidative Stress

摘要

Our results demonstrate, for the first time, that ONE metabolites, in addition to HNE metabolites, are formed in vivo as products of LPO. Our semi-quantitative data (normalized peak areas), suggest that oxidation of the aldehyde functionality in the HNE and ONE conjugates is the preferred metabolic pathway because HNA-MA lactone and ONA-MA are among the most abundant end products of HPNE metabolism. Our findings indicate that the end products of HPNE metabolism are elevated in urine obtained from CCl_(4)-treated rats, which holds promise for these metabolites as markers of oxidative stress in animals and humans.