Method for Dissolution Profile Determination of Probiotic Bacteria from Controlled Release Solid Oral Dosage Forms via Direct Enumeration Fluorochrome Staining

摘要

4',6-diamidino-2-phenylindole (DAPI) fluorochrome stained probiotic bacteria dissolution samples were used to directly enumerate and determine release profiles from controlled release solid oral dosage forms. Combined with indirect agar plate counting techniques, this method allows for quantification of viable bacterial proportion surviving dosage form manufacture and simulated gastric exposure.