In vitro maturation of human ova

摘要

The basis of IVM is the maturing in vitro of oocytes from Germinal Vesicle (GV) stage of development to the metaphase II stage Oocytes are retrieved for IVM from antral follicles 2-10 mm diameter and matured in vitio for 24 to 52 h A high proportion of the in vitro matured oocytes is able to resume meiosis and reach the Mil stage in vitro. Their ability to be fertilized after 48-56 h of IVM is also high and a similar proportion is able to undergo early cleavage stage development comparable with conventional IVF and ICSI embryos It has been questioned whether in vitro matured human oocytes are intrinsically compromised or whether culture conditions are inadequate to support the developmental capacity of the oocytes However, recent data taken together suggest that in future immature oocyte retrieval combined with IVM could possibly replace standard stimulated IVF in selected patients Women suffering from polycystic ovarian syndrome (PCOS) are extremely sensitive to stimulation with exogenous gonadotrophins in assisted reproduction, and they have a significant risk of developing OHSS An attractive alternative to ovarian stimulation in women with PCOS would therefore be to retrieve immature oocytes Immature oocyte retrieval combined with IVM could also replace standard stimulation in healthy regularly cycling women referred for IVF (ICSI) due to severe male infertility. In both groups, clinical pregnancy rates of 24% per aspiration has been obtained The reported children born after IVM are healthy