Regulation of the human taurine transporter by TNF-α and an anti-inflammatory function of taurine in human intestinal Caco-2 cells

摘要

We investigated whether or not the inflammatory cytokines affect the activity of taurine transporter (TAUT) in human intestinal Caco-2 cells. Among the cytokines, tumor necrosis factor α(TNF-α) markedly augmented the TAUT activity. A kinetic analysis of the TAUT activity in TNF-α-treated Caco-2 cells suggests that this up-regulation was associated with both an increase in the amount of TAUT and an increase in its affinity. Considering these results, it seems that intracellular taurine plays a role in the intestinal epithelial cells under such an inflammatory condition as that caused by an excessive amount of TNF-α secreted by macrophages. To verify this hypothesis, we examined the effect of taurine on inflamed intestinal cells by using a co-culture system of Caco-2 cells with human macrophage-like THP-1 cells. The result shows that taurine significantly repressed the damage to Caco-2 cells caused by TNF-α secreted by THP-1 cells. Thus, taurine may be a useful substance against intestinal inflammation.