Screen and Qualitatively Detect Ingredient of Genetically Modified Soybean by Multiplex Fast-PCR

摘要

Designing and screening suitable primers for amplifying CaMY35S gene and soybean lectin gene by multiplex PCR, which could detect if foodstuffs exist ingredients of genetically modified (GM) soybean. The time of multiplex PCR was finished within 50 minutes; Designing and screening four primers for amplifying promoter CaMV35S - petunia CTP4 gene and cp4-epsps gene respectively, which only exist in genetically modified soybean Roundup Ready. This multiplex PCR could detect if there existed ingredient of GM soybean Roundup Ready in foodstuffs within 70 minutes. Not only could screen GM soybean but also could detect GM soybean Roundup Ready by two multiplex PCR system. The methods could not only reduce the time of detect and expense, but improve efficiency of job as well.