Orthogonal chemical ligation for biomolecular engineering

摘要

Chemoselective ligation of unprotected peptides has established itself as a method for the synthesis of functional biological macromolecules,including engineered peptides and proteins.In the native chemical ligation method in particular [1],a C-treminal peptide thioester reacts with an N-terminal cysteine peptide to form an amide bond at the juntion point.