New insights into the membrane translocating process of the tat peptide used for cellular delivery of various biological molecules

摘要

A 14-mer peptide derived from the HIV Tat protein was shown to translocate efficiently through the plasma membrane.Basic amino acid residues in this highly cationic peptide are responsible for its internalization.Non-permeant peptides,antisense oligonucleotides,proteins and even particulate material can be efficiently internalized when chemically conjugated or genetically fused to this Tat peptide.However,the mechanism of this uptake still remains to be elucidated.