Structure elucidation of peptides with unnatural amino acids using an automated protein sequencer

摘要

The use of automatic Edman microsequencing to determine the primary structure of peptides or proteins containing 20 eukaryotic natural amino acids is now routine.However,the ability to readily sequence peptides containing multiple unnatural amino acids remains elusive.We recently reported on the elution profile of 35 PTH-derivatives of unnatural alpha-amino acids on an ABI microsequencer.