Optimization of Extraction Conditions for Glycosaminoglycan from Urechis unicinctus by Response Surface Methodology

摘要

The enzyme dosage, enzymolysis time, and alcohol precipitation concentration as independent variables and the extraction rate as the response value, the extraction technology of glycosaminoglycan from Urechis unicinctus was optimized via response surface methodology on the basis of single-factor experiments. The extraction rate of glycosaminoglycan was affected in the following order: concentration of alcohol precipitation ＞ enzymolysis time ＞ enzyme dosage. The optimal conditions for the glycosaminoglycan extraction were as follows: alcohol precipitation concentration 75 %, enzymolysis time 4 h, and enzyme dosage 0.4 %.