Optimized environmental conditions for petroleum degradation of strain B1 by orthogonal test

摘要

A strain B1 belonging to Pseudomonas aeruginosa with high petroleum-degrading efficiency was isolated from Dagang oil-field wastewater treatment plant.More than 80％ petroleum-degrading efficiency was obtained at 32°C,200 r/min.According to the orthogonal test,the environmental impact factors influencing petroleum-degrading efficiency of the strain were investigated.The optimum environmental conditions for petroleum degradation were as follows:temperature was 40 °C,pH 8.0,oil inoculation 1 mL,nitrogen content 0.05 g.And the biodegradation efficiency of the strain reached 86.1％.