Antioxidant status and lipid peroxidation in the muscle of German Simmental and German Holstein bulls fed n-3 and n-6 PUFA-based diets

摘要

The studies were established to investigate effects of m-3 and n-6 PUFA-based diets on the relationship between antioxidant status and lipid peroxidation in beef muscle. Two indoor feeding experiments with German Simmental and German Holstein bulls were carried out premised on m-3 PUFA based (grass silage) and n-6 PUFA based diets (maize silage) with supplements. Antioxidant capacity (TEAC, FRAP), antioxidant enzymes, TBARS and fatty acid profiles were investigated. The antioxidant capacity of muscle of both breeds using TEAC and FRAP assay was not affected by different PUFA-based diets, however m-3 PUFA based diet caused a significantly higher extent of lipid peroxidation using TBARS assay in muscle of German Simmental- and German Holstein bulls compared to n-6 PUFA based diets. Muscle of the m-3 PUFA based diets showed higher activity of catalase of both breeds, and additional elevated superoxide dismutase activity in German Simmental bulls compared to muscle of bulls fed n-6 PUFA based diets. N-3 PUFA based diet caused increased level of all single m-3 fatty acids resulting in decreased m-6/m-3 PUFA ratio in muscle of both breeds.