Role of shear and extensional stress on the degradation of proteins during downstream processing

摘要

In most applications of biotechnology and downstream processing proteins are stressed invarious flow configurations by shear and extensional stress. In this work we present stressexperiments of several proteins under well-defined flow conditions. In combination with insituanalytics flow-induced aggregation phenomena and conformational changes can besuccessfully monitored. In addition to shear and elongation, the contribution of adsorptionphenomena to interfaces is investigated.