Heterologous Expression of Two Ferulic Acid Esterasesfrom Penicillium funiculosum

摘要

Two recombinant ferulic acid esterases from Penicillium funiculosum producedin Aspergillus awamori were evaluated for their ability to improve the digestibility ofpretreated corn stover. The genes, faeA and faeB, were cloned from P. funiculosum andexpressed in A. awamori using their native signal sequences. Both enzymes contain acatalytic domain connected to a family 1 carbohydrate-binding module by a threonine-richlinker peptide. Interestingly, the carbohydrate binding-module is N-terminal in FaeA andC-terminal in FaeB. The enzymes were purified to homogeneity using column chromatog-raphy, and their thermal stability was characterized by differential scanning microcalorimetry.We evaluated both enzymes for their potential to enhance the cellulolytic activity of purifiedTrichoderma reesei Cel7A on pretreated corn stover.