Infrared Light Induced Patterning of Proteins on ppNIPAM Thermoresponsive Thin Films: A amp;#8220;Protein Laser Printeramp;#8221;

摘要

Controlled adsorption and patterning of proteins on a thin film of thermo-responsive plasma-deposited poly-N-isopropylacrylamide occurs in response to heating with a computer-controlled infrared laser beam, with pattern resolution in the single micrometer range and adsorption times within a few seconds. Deposition of multiple proteins in the same substrate region has been accomplished. Successful streptavidin-biotin and antibody-antigen binding indicates that protein function stays intact after proteins adsorb to the ppNIPAM bio-polymer surface.