The inhibitory activity of Lignosulfonic acid sodium on HIV-1 infection

摘要

Currently there are 25 drugs approved on the market for people who live with HIV-1. These drugs target various stages of the viral life cycle, mainly viral entry, fusion and reverse transcription[1]. As with all drugs, all of them have side-effects and are subject to drug-resistant mutations. Therefore, consistent efforts are needed for the search of new drug targets. In this report, using an in vitro pseudovirus assay, we were able to analyze the anti-HIV-1 activity of Lignosulfonic acid sodium. Lignosulfonic acid sodium salt (LSA) is usually used as industry material, but we found that it exhibited anti-HIV-1 activity. LSA exhibited potent HIV-1 inhibitory activities in a dose-dependent manner. In addition, we investigated the cytotoxcity of LSA using MTT colarimetric assay and determined the 50% toxic dose (CC50) to be at 132μg/mL. When the concentration of LSA is 9μg/mL, which is much lower than CC50, the inhibition ratio of pseudotyped HIV-1 virus still more than 90%. In this study, we also showed that pseudotyped virus could be rapidly generated and used in a number of established cell lines with low background[2], and adapted for evaluating anti-viral agents that act on the viral entry portal.