DETERMINATION OF PCDDS/DFS AND DIOXIN-LIKE PCBS IN EGG AND FISH FEED SAMPLES IN TAIWAN BY THE DR CALUX(R) BIOASSAY

摘要

After an in-depth training and a 3-phase cross-validation by staff of the BioDetection Systems, we obtained the licence for the DR CALUX(R) bioassay. The control charts were initially set up by using 1 pM and 3 pM 2,3,7,8-TCDD and two certified reference materials for quality assurance and quality control (QA/QC) of performing the DR CALUX(R)bioassay in our laboratory. Thirty-one egg, six eel-feed, nine seabass-feed, and eight tilapia-feed samples were collected and their fat contents were determined to be 10.3 ± 1.1%, 9.2 ± 1.0%,11.5 ± 2.5%, and 8.2 ± 1.3%, respectively. By the DR CALUX bioassay, dioxins and dioxin-like compounds were determined to be 2.0 ± 0.7 pg DR CALUX-TEQ/g fat, 0.4 ± 0.1 ng DR CALUX-TEQ/kg product, 0.9 ± 0.6 ng DR CALUX-TEQ/kg product, and 0.3 ± 0.1 ng DR CALUX-TEQ/kg product, respectively, which were all below the EU-limits for egg and fish feeds.