Novel algorithms in coherent diffraction imaging using x-ray free-electron lasers

摘要

The emergence of X-ray Free-Electron Lasers has enabled coherent diffraction imaging of single nanoparticles byoutrunning radiation damage with an intense ultrafast X-ray pulse. A 3D reconstruction from an ensemble of 2Ddiffraction patterns requires recovery of the orientations of individual diffraction patterns. This assumes that eachdiffraction pattern comes from a slice of a common diffraction volume. In the presence of particle heterogeneity, thisassumption does not hold and the recovered structure is severely degraded. In this paper, I review couple of emergingalgorithms useful for dealing with conformational changes in a heterogeneous sample. A simulated case study of a“particle in motion” is included to demonstrate the algorithms and also show that these novel algorithms work in thepresence of missing Fourier regions caused by new detector geometries at XFEL facilities.© (2012) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.