On-demand DNA sensor with ligand fixing method

摘要

It is well known that the DNA sequence carries the genetic information. The DNA sequencing and detection is now the central issues of clinical research and a DNA sensor chip is indispensable device. There are many sensing methods, the EIS is a highly sensitive, label free and small ions-strength dependence method. The surface charge transfer resistance (Rct), calculated using the Randles equivalent circuit with Warburg corresponds to the DNA concentration. For the selectivity, probe-DNA which has complimentary sequence to the target DNA is usually fixed on the sensor surface. Once surface is modified, the device is defined for the specific target DNA. To avoid such inflexibility, fresh probe-DNA modification at point of care is needed. To achieve such on-demand DNA chip, we propose a new probe-DNA fixation (Fig. 1).