On-chip ultraviolet holography for high-throughput nanoparticle and biomolecule detection

机译：片上紫外线全息技术，用于高通量纳米粒子和生物分子检测

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Nanoparticle and biomolecule imaging has become an important need for various applications. In an effort to find a higher throughput alternative to existing devices, we have designed a lensfree on-chip holographic imaging platform operating at an ultraviolet (UV) wavelength of 266 nm. With a custom-designed free-space light delivery system to illuminate the sample that is placed very close (＜0.5 mm) to an opto-electronic image sensor chip, without any imaging lenses in between, the full active area of the imager chip (＞16 mm ) was utilized as the imaging field-of-view (FOV) capturing holographic signatures of target objects on a chip. These holograms were then digitally back propagated to extract both the amplitude and phase information of the sample. The increased forward scattering from nanoparticles due to this shorter illumination wavelength has enabled us to image individual particles that are smaller than 30 nm over an FOV of ＞16 mm~2. Our platform was further utilized in high-contrast imaging of nanoscopic biomolecule aggregates since 266 nm illumination light is strongly absorbed by biomolecules including proteins and nucleic acids. Aggregates of Cu/Zn-superoxide dismutase (SOD1), which has been linked to a fatal neurodegenerative disease, ALS (amyotrophic lateral sclerosis), have been imaged with significantly improved contrast compared to imaging at visible wavelengths. This unique UV imaging modality could be valuable for biomedical applications (e.g., viral load measurements) and environmental monitoring including air and water quality monitoring.

机译：纳米颗粒和生物分子成像已成为各种应用的重要需求。为了找到更高吞吐量的现有设备替代产品，我们设计了一种无透镜的芯片上全息成像平台，该平台可在266 nm的紫外线波长下工作。借助定制设计的自由空间光传输系统，可以照亮非常靠近光电图像传感器芯片（小于0.5毫米）放置的样品（小于0.5毫米），而在它们之间没有任何成像透镜，则成像器芯片的整个有效区域（＞ 16 mm）被用作成像视场（FOV），用于捕获芯片上目标物体的全息签名。然后将这些全息图进行数字反向传播，以提取样品的振幅和相位信息。由于较短的照射波长，纳米颗粒的前向散射增加，使我们能够在大于16 mm〜2的FOV上成像小于30 nm的单个颗粒。我们的平台进一步用于纳米生物分子聚集体的高对比度成像，因为266 nm的照明光被蛋白质和核酸等生物分子强烈吸收。与可见光波长成像相比，已与致命性神经退行性疾病ALS（肌萎缩性侧索硬化）相关的Cu / Zn-超氧化物歧化酶（SOD1）聚集体成像。这种独特的UV成像方式对于生物医学应用（例如病毒载量测量）和环境监测（包括空气和水质监测）可能有价值。

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《Optics and Biophotonics in Low-Resource Settings IV》|2018年|1048510.1-1048510.6|共6页
会议地点 San Francisco(US)
作者
Mustafa Ugur Daloglu; Aniruddha Ray; Zoltan Gorocs; Matthew Xiong; Ravinder Malik; Gal Bitan; Euan McLeod; Aydogan Ozcan;
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Electrical and Computer Engineering Department, University of California, Los Angeles, CA, 90095, USA,Bioengineering Department, University of California, Los Angeles, CA, 90095, USA,California NanoSystems Institute (CNSI), University of California, Los Angeles, CA, 90095, USA;

Electrical and Computer Engineering Department, University of California, Los Angeles, CA, 90095, USA,Bioengineering Department, University of California, Los Angeles, CA, 90095, USA,California NanoSystems Institute (CNSI), University of California, Los Angeles, CA, 90095, USA;

Electrical and Computer Engineering Department, University of California, Los Angeles, CA, 90095, USA,Bioengineering Department, University of California, Los Angeles, CA, 90095, USA,California NanoSystems Institute (CNSI), University of California, Los Angeles, CA, 90095, USA;

Electrical and Computer Engineering Department, University of California, Los Angeles, CA, 90095, USA;

Department of Neurology, David Geffen School of Medicine, University of California, Los Angeles, CA, 90095, USA;

Department of Neurology, David Geffen School of Medicine, University of California, Los Angeles, CA, 90095, USA,Brain Research Institute, University of California, Los Angeles, CA, 90095, USA,Molecular Biology Institute, University of California, Los Angeles, CA, 90095, USA;

College of Optical Sciences, University of Arizona, Tucson, AZ 85721, USA;

Electrical and Computer Engineering Department, University of California, Los Angeles, CA, 90095, USA,Bioengineering Department, University of California, Los Angeles, CA, 90095, USA,California NanoSystems Institute (CNSI), University of California, Los Angeles, CA, 90095, USA,Department of Surgery, David Geffen School of Medicine, University of California, Los Angeles, CA, 90095, USA;

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正文语种 eng
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关键词
Digital holography; on-chip imaging; medical and biological imaging;

机译：数字全息术；片上成像；医学和生物成像;

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专利

1. Computational On-Chip Imaging of Nanoparticles and Biomolecules using Ultraviolet Light [J] . Mustafa Ugur Daloglu, Aniruddha Ray, Zoltan Gorocs, Scientific reports. . 2017,第1期

机译：使用紫外光计算纳米颗粒和生物分子的计算片上成像
2. On-chip hydrodynamic chromatography separation and detection of nanoparticles and biomolecules [J] . Blom MT., Chmela E., Oosterbroek RE., Analytical chemistry . 2003,第24期

机译：片上流体动力学色谱法分离和检测纳米颗粒和生物分子
3. Aluminum Nanoparticles as Substrates for Metal-Enhanced Fluorescence in the Ultraviolet for the Label-Free Detection of Biomolecules [J] . Mustafa H. Chowdhury, Krishanu Ray, Stephen K. Gray, Analytical chemistry . 2009,第4期

机译：铝纳米粒子作为基质的紫外线中金属增强荧光的无标记生物分子检测
4. On-chip ultraviolet holography for high-throughput nanoparticle and biomolecule detection [C] . Mustafa Ugur Daloglu, Aniruddha Ray, Zoltan Gorocs, SPIE Conference on Optics and Biophotonics in Low-Resource Settings . 2018

机译：用于高通量纳米粒子和生物分子检测的片上紫外全息
5. High-Throughput and Simultaneous Analysis of 12 Cannabinoids in Cannabis Products Using Liquid Chromatography with Ultraviolet Detection [D] . Pathipaka, Shashi Bhushan 2018

机译：高效液相色谱-紫外检测同时分析大麻产品中的12种大麻素
6. Computational On-Chip Imaging of Nanoparticles and Biomolecules using Ultraviolet Light [O] . Mustafa Ugur Daloglu, Aniruddha Ray, Zoltan Gorocs, -1

机译：使用紫外光的纳米粒子和生物分子的计算芯片成像。
7. Computational On-Chip Imaging of Nanoparticles and Biomolecules using Ultraviolet Light [O] . Daloglu, Mustafa Ugur, Ray, Aniruddha, Gorocs, Zoltan, 2017

机译：使用紫外光的纳米粒子和生物分子的计算芯片成像。

On-chip ultraviolet holography for high-throughput nanoparticle and biomolecule detection

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