Improving the stability of cellulase by immobilization on chitosan-coated magnetic nanoparticles modified with α-ketoglutaric acid

摘要

The application of cellulases in various industries demands highly stable enzymes, able to perform at extreme pH values and temperatures. In this study, the cellulase, which was immobilized on the chitosan-coated magnetic nanoparticles modified with α-ketoglutaric acid (α-KA-CCMNPs), exhibited a broader pH range of high activity and the loss of the activity of immobilized cellulase was lower than that of free cellulase. And the optimal temperature for immobilized cellulase was 50 ℃, whereas that for free cellulase was 40 ℃. Meanwhile, the immobilized cellulase showed better thermal stability than free cellulase at high temperature range. In addition, the storage stability of the cellulase was improved greatly after it was immobilized on the surface of α-KA-CCMNPs.