In an attempt to improve glutathione content in Saccharomyces cerevisiae，the haploid strain(a)was constructed by genetic homologous recombination，in which the homocysteinemethyl-transferase(MET6)gene was disrupted. The mutant strain was tested on 5 l fermentorcompared with that of the parent strain，and the results showed an obvious increase of glutathionecontent. The final glutathione yield of the mutant reached 1656 mg/l with 40% increasementcompared to the parent. Therefore，these approaches uncover the possibility of knocking outMET6 gene and increasing glutathione yield during fermentation.
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