Second ion source for lockmass calibration of matrix assisted laser desorption ionisation mass spectrometer

摘要

A mass spectrometer or ion imaging apparatus comprising: a first ion source for acquiring an ion image of a target, whereby separate mass spectral data corresponding to different regions and/or different depths of the target are acquired; and a second ion source for generating calibrant, lockmass or reference ions. Also disclosed are methods of mass spectrometry and ion imaging using the above apparatuses. The second ion source is for generating calibrant, lockmass or reference ions. The second ion source may be arranged downstream of the first ion source, such that both the analyte and calibrant ions are detected simultaneously.