METHOD FOR DETECTING OFF-TARGET EFFECT OF ADENINE BASE EDITOR SYSTEM BASED ON WHOLE-GENOME SEQUENCING AND USE THEREOF IN GENE EDITING

摘要

The present invention provide a method for detecting the genome-wide off-target effects of adenine base editor (ABE) and the application in gene editing thereof. ABE comprises the TadA:TadA*:Cas9 fusion protein and gRNA which is able to catalyze the substitution of A to G with high efficiency at the target site, which can bring ABE a bright application prospect in gene editing and construction of disease model for human disease. Thus, the present invention provides the EndoV-seq method first time to detect the genome-wide off-target effects of ABE. The EndoV-seq method has a wide application prospect in gene editing, especially in gene editing for treatment field of human disease.